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    SEC Form 10-Q filed by Twist Bioscience Corporation

    8/4/25 4:24:11 PM ET
    $TWST
    Biotechnology: Biological Products (No Diagnostic Substances)
    Health Care
    Get the next $TWST alert in real time by email
    twst-20250630
    false0001581280--09-302025Q3Subsequent Event
    [PLACEHOLDER]

    * * * * *
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    Table of Contents
        
    UNITED STATES
    SECURITIES AND EXCHANGE COMMISSION
    Washington, D.C. 20549
    FORM 10-Q
    (Mark One)
    ☒QUARTERLY REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934

    For the quarterly period ended June 30, 2025
    OR

    ☐TRANSITION REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934
    For the transition period from to
    Commission File Number: 001-38720
    twsti log .jpg
    Twist Bioscience Corporation
    (Exact Name of Registrant as Specified in its Charter)

    Delaware46-2058888
    (State or other jurisdiction of(I.R.S. Employer
    incorporation or organization)Identification No.)

    681 Gateway Blvd, South San Francisco, CA 94080
    (Address of principal executive offices and zip code)
    (800) 719-0671
    (Registrant’s telephone number, including area code)

    Title of each classTrading Symbol(s)Name of each exchange on which registered
    Common StockTWSTThe Nasdaq Global Select Market

    Indicate by check mark whether the Registrant: (1) has filed all reports required to be filed by Section 13 or 15(d) of the Securities Exchange Act of 1934 during the preceding 12 months (or for such shorter period that the Registrant was required to file such reports), and (2) has been subject to such filing requirements for the past 90 days. Yes ☒ No ☐

    Indicate by check mark whether the Registrant has submitted electronically every Interactive Data File required to be submitted pursuant to Rule 405 of Regulation S-T (§232.405 of this chapter) during the preceding 12 months (or for such shorter period that the Registrant was required to submit such files). Yes ☒ No ☐

    Indicate by check mark whether the Registrant is a large accelerated filer, an accelerated filer, a non-accelerated filer, a smaller reporting company, or an emerging growth company. See the definitions of “large accelerated filer,” “accelerated filer,” “smaller reporting company” and “emerging growth company” in Rule 12b-2 of the Exchange Act. (Check one):



    Table of Contents
    Large accelerated filer☒Accelerated filer☐
    Non-accelerated filer☐Smaller reporting company☐
      Emerging growth company☐
    If an emerging growth company, indicate by check mark if the registrant has elected not to use the extended transition period for complying with any new or revised financial accounting standards provided pursuant to Section 13(a) of the Exchange Act. ☐

    Indicate by check mark whether the Registrant is a shell company (as defined in Rule 12b-2 of the Exchange Act). Yes ☐ No ☒

    APPLICABLE ONLY TO ISSUERS INVOLVED IN BANKRUPTCY PROCEEDINGS DURING THE PRECEDING FIVE YEARS:

    Indicate by check mark whether the registrant has filed all documents and reports required to be filed by Section 12, 13 or 15(d) of the Securities Exchange Act of 1934 subsequent to the distribution of securities under a plan confirmed by a court. Yes ☐ No ☐

    APPLICABLE ONLY TO CORPORATE ISSUERS

    Indicate the number of shares outstanding of each of the issuer’s classes of common stock, as of the latest practicable date.

    The number of shares of the Registrant’s common stock outstanding as of July 30, 2025, was 60,360,925.



    Table of Contents
    TWIST BIOSCIENCE CORPORATION
    QUARTERLY REPORT ON FORM 10-Q
    FOR THE QUARTER ENDED JUNE 30, 2025
    TABLE OF CONTENTS
    FORWARD-LOOKING STATEMENTS
    2
    PART I. FINANCIAL INFORMATION
    4
    Item 1.
    Financial Statements (unaudited)
    4
    Condensed Consolidated Balance Sheets (unaudited)
    4
    Condensed Consolidated Statements of Operations and Comprehensive Income (Loss) (unaudited)
    5
    Condensed Consolidated Statements of Stockholders’ Equity (unaudited)
    6
    Condensed Consolidated Statements of Cash Flows (unaudited)
    8
    Notes to Unaudited Condensed Consolidated Financial Statements
    10
    Item 2.
    Management’s Discussion and Analysis of Financial Condition and Results of Operations
    27
    Item 3.
    Quantitative and Qualitative Disclosures About Market Risk
    36
    Item 4.
    Controls and Procedures
    36
    PART II. OTHER INFORMATION
    37
    Item 1.
    Legal Proceedings
    37
    Item 1A.
    Risk Factors
    37
    Item 2.
    Unregistered Sales of Equity Securities and Use of Proceeds
    37
    Item 3.
    Defaults Upon Senior Securities
    37
    Item 4.
    Mine Safety Disclosures
    37
    Item 5.
    Other Information
    37
    Item 6.
    Exhibits
    39
    Signatures
    41



    1

    Table of Contents
    FORWARD-LOOKING STATEMENTS
    This Quarterly Report on Form 10-Q for the quarter ended June 30, 2025 (“Form 10-Q”) contains forward-looking statements within the meaning of Section 27A of the Securities Act of 1933, as amended, or the Securities Act, and Section 21E of the Securities Exchange Act of 1934, as amended, or the Exchange Act. These statements relate to, among other matters, future growth, expansion and other expectations regarding future operations plans and financial performance. Forward-looking statements are also identified by the words “believe,” “will,” “may,” “estimate,” “continue,” “anticipate,” “intend,” “should,” “plan,” “expect,” “predict,” “could,” “potentially” and variations of such words and similar expressions. You should not rely upon forward-looking statements as predictions of future events. Such statements are based on management’s expectations as of the date of this filing and involve many risks and uncertainties that could cause our actual results, events or circumstances to differ materially from those expressed or implied in our forward-looking statements. Such risks and uncertainties include:
    •our ability to increase our revenue and our revenue growth rate;
    •our ability to accurately estimate capital requirements and our needs for additional financing;
    •our estimates of the size of our market opportunities;
    •our ability to increase DNA production, reduce turnaround times and drive cost reductions for our customers;
    •our ability to effectively manage our growth;
    •our ability to successfully enter new markets and manage our international expansion;
    •our ability to develop and commercialize additional products in the synthetic biology and biologic drug industries;

    •our ability to leverage our investment in our manufacturing facility in Wilsonville, Oregon;

    •our ability to protect our intellectual property, including our proprietary DNA synthesis platform;
    •costs associated with defending intellectual property infringement and other claims;
    •the effects of increased competition in our business;
    •our ability to keep pace with changes in technology and our competitors;
    •our ability to successfully identify, evaluate and manage any future acquisitions of businesses, solutions or technologies;
    •the success of our marketing efforts;
    •a significant disruption in, or breach in security of our information technology systems and resultant interruptions in service and any related impact on our reputation;
    •our ability to attract and retain qualified employees and key personnel;
    •the effects of natural or man-made catastrophic events or public health emergencies;
    •the effectiveness of our internal controls;
    •changes in government regulation affecting our business;
    •uncertainty as to economic and market conditions and the impact of adverse economic conditions; and
    •other risk factors included under the section titled “Risk factors” contained in Part I, Item 1A of our Annual Report on Form 10-K filed with the SEC on November 18, 2024 (the "Annual Report on Form 10-K"), and Part II, Item 1A of our Quarterly Report on Form 10-Q filed with the SEC on May 5, 2025.
    2

    Table of Contents
    You should not rely upon forward-looking statements as predictions of future events. Such statements are based on management’s expectations as of the date of this filing and involve many risks and uncertainties that could cause our actual results, events or circumstances to differ materially from those expressed or implied in our forward-looking statements.
    Readers are urged to carefully review and consider all of the information in this Form 10-Q and in other documents we file from time to time with the Securities and Exchange Commission, or SEC. We undertake no obligation to update any forward-looking statements made in this Form 10-Q to reflect events or circumstances after the date of this filing or to reflect new information or the occurrence of unanticipated events, except as required by law. We may not actually achieve the plans, intentions or expectations disclosed in our forward-looking statements. Our forward-looking statements do not reflect the potential impact of any future acquisitions, mergers, dispositions, joint ventures or investments we may make.
    When we use the terms “Twist,” “Twist Bioscience,” the “Company,” “we,” “us” or “our” in this report, we are referring to Twist Bioscience Corporation and its consolidated subsidiaries unless the context requires otherwise. Sequence space and the Twist logo are trademarks of Twist Bioscience Corporation. All other company and product names may be trademarks of the respective companies with which they are associated.



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    PART I. FINANCIAL INFORMATION

    Item 1. Financial Statements

    Twist Bioscience Corporation
    Condensed Consolidated Balance Sheets (unaudited)
    (In thousands, except per share data)June 30,
    2025
    September 30,
    2024
    Assets  
    Current assets: 
    Cash and cash equivalents $201,373 $226,316 
    Short-term investments49,42550,083
    Accounts receivable, net [1]
    49,85434,903
    Inventories25,93824,078
    Prepaid expenses and other current assets [2]
    13,18611,396
    Total current assets$339,776 $346,776 
    Property and equipment, net95,351102,520
    Operating lease right-of-use assets51,84558,829
    Investment in equity securities54,3371,525
    Goodwill82,19585,811
    Intangible assets, net13,68814,478
    Restricted cash, non-current2,5322,816
    Other non-current assets [3]
    3,8811,568
    Total assets$643,605 $614,323 
    Liabilities and stockholders’ equity
    Current liabilities:
    Accounts payable$11,985 $1,630 
    Accrued expenses19,53515,104
    Accrued compensation28,97233,650
    Current portion of operating lease liability15,50114,805
    Other current liabilities11,0775,817
    Total current liabilities$87,070 $71,006 
    Operating lease liability, net of current portion62,64970,221
    Liability related to the sale of future revenue15,000—
    Other non-current liabilities[4]
    688407
    Total liabilities $165,407 $141,634 
    Commitments and contingencies (Note 10)
    Stockholders’ equity
    Common stock, $0.00001 par value —200,000 and 100,000 shares authorized at June 30, 2025 and September 30, 2024, respectively; 60,174 and 58,877 shares issued and outstanding at June 30, 2025 and September 30, 2024, respectively
    $— $— 
    Additional paid-in capital1,771,5021,715,119
    Accumulated other comprehensive income/(loss)(864)(522)
    Accumulated deficit(1,292,440)(1,241,908)
    Total stockholders’ equity$478,198 $472,689 
    Total liabilities and stockholders’ equity$643,605 $614,323 
    [1] Including related parties' balances of $1.2 million and $0.5 million as of June 30, 2025 and September 30, 2024, respectively.
    [2] Including related party balances of $1.7 million and $— as of June 30, 2025 and September 30, 2024, respectively.
    [3] Including related party balances of $1.7 million and $— as of June 30, 2025 and September 30, 2024, respectively.
    [4] Including related party balances of $0.2 million and $— as of June 30, 2025 and September 30, 2024, respectively.
    The accompanying notes are an integral part of these unaudited condensed consolidated financial statements.
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    Twist Bioscience Corporation
    Condensed Consolidated Statements of Operations and Comprehensive Income (Loss) (unaudited)

    Three Months Ended
    June 30,
    Nine Months Ended
    June 30,
    (In thousands, except per share data)2025202420252024
    Revenues [1]
    $96,057 $81,464 $277,563 $228,264 
    Costs and expenses:
    Cost of revenues$44,760 $46,193 $137,398 $133,148 
    Research and development expenses18,04722,46963,27169,718
    Selling, general and administrative expenses63,37056,794183,219165,256
    Impairment of long-lived assets— 44,930 — 44,930 
    Total costs and expenses$126,177 $170,386 $383,888 $413,052 
    Loss from operations$(30,120)$(88,922)$(106,325)$(184,788)
    Interest income$2,690 $3,663 8,73111,724
    Gain on sale of business 48,847—48,847—
    Other income (expense), net [2]
    (836)(121)(1,323)(351)
    Income (loss) before income taxes$20,581 $(85,380)$(50,070)$(173,415)
    Income tax expense(191)(191)(462)(656)
    Net income (loss)$20,390 $(85,571)$(50,532)$(174,071)
    Other comprehensive income (loss):
    Change in unrealized gain (loss) on investments$(25)$(21)$(122)$56 
    Foreign currency translation adjustment(177)(54)(220)(35)
    Comprehensive income (loss)$20,188 $(85,646)$(50,874)$(174,050)
    Basic earnings per share:
    Net income (loss) per share, basic$0.34 $(1.47)$(0.85)$(3.01)
    Weighted average shares used in computing net income (loss) per share, basic59,99558,14559,60057,806
    Diluted earnings per share:
    Net income (loss) per share, diluted$0.33 $(1.47)$(0.85)$(3.01)
    Weighted average shares used in computing net income (loss) per share, diluted61,16458,14559,60057,806
    [1] Including revenues from related parties of $2.8 million and $9.6 million during the three and nine months ended June 30, 2025, respectively and $4.1 million and $9.7 million during the three and nine months ended June 30, 2024, respectively.
    [2] Including sublease income from a related party of $0.4 million and $— for the three and nine months ended June 30, 2025 and June 30, 2024, respectively.

    The accompanying notes are an integral part of these unaudited condensed consolidated financial statements.

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    Twist Bioscience Corporation
    Condensed Consolidated Statements of Stockholders’ Equity (unaudited)

    Three Months Ended June 30, 2025
    Common
    stock
    Additional
    paid-in
    capital
    Accumulated
    Other
    comprehensive
    loss
    Accumulated
    deficit
    Total
    stockholders'
    equity
    (In thousands)SharesAmount
    Balances as of March 31, 202559,857$— $1,753,600 $(662)$(1,312,830)$440,108 
    Vesting of restricted stock units227$— $— $— $— $— 
    Exercise of stock options90—1,829——1,829
    Repurchases of common stock for income tax withholding——(3)——(3)
    Stock-based compensation——16,076——16,076
    Other comprehensive loss———(202)—(202)
    Net income————20,39020,390
    Balances as of June 30, 202560,174$— $1,771,502 $(864)$(1,292,440)$478,198 

    Three Months Ended June 30, 2024
    Common
    stock
    Additional
    paid-in
    capital
    Accumulated
    Other
    comprehensive
    income
    Accumulated
    deficit
    Total
    stockholders'
    equity
    (In thousands)SharesAmount
    Balances as of March 31, 202458,162$— $1,682,473 $(660)$(1,121,682)$560,131 
    Vesting of restricted stock units223$— $— $— $— $— 
    Exercise of stock options134—2,915——2,915
    Repurchases of common stock for income tax withholding——(7)——(7)
    Stock-based compensation——13,746——13,746
    Other comprehensive loss———(75)—(75)
    Net loss————(85,571)(85,571)
    Balances as of June 30, 202458,519$— $1,699,127 $(735)$(1,207,253)$491,139 
    The accompanying notes are an integral part of these unaudited condensed consolidated financial statements.

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    Nine Months Ended June 30, 2025
    Common
    stock
    Additional
    paid-in
    capital
    Accumulated
    Other
    comprehensive
    income
    Accumulated
    deficit
    Total
    stockholders'
    equity
    (In thousands)SharesAmount
    Balances as of September 30, 202458,877$— $1,715,119 $(522)$(1,241,908)$472,689 
    Restricted common stock canceled(137)$— $— $— $— $— 
    Vesting of restricted stock units1,038—————
    Exercise of stock options331—5,551——5,551
    Issuance of shares under the employee stock purchase plan65—2,429——2,429
    Repurchases of common stock for income tax withholding——(14)——(14)
    Stock-based compensation——48,417——48,417
    Other comprehensive loss———(342)—(342)
    Net loss————(50,532)(50,532)
    Balances as of June 30, 202560,174$— $1,771,502 $(864)$(1,292,440)$478,198 

    Nine Months Ended June 30, 2024
    Common
    stock
    Additional
    paid-in
    capital
    Accumulated
    Other
    comprehensive
    income
    Accumulated
    deficit
    Total
    stockholders'
    equity
    (In thousands)SharesAmount
    Balances as of September 30, 202357,557$— $1,657,222 $(756)$(1,033,034)$623,432 
    Impact of ASU 2016-13 adoption—$— $— $— $(148)$(148)
    Vesting of restricted stock units712—————
    Exercise of stock options261—5,183——5,183
    Issuance of shares under the employee stock purchase plan124—2,047——2,047
    Repurchases of common stock for income tax withholding(135)—(3,967)——(3,967)
    Stock-based compensation——38,642——38,642
    Other comprehensive income———21—21
    Net loss————(174,071)(174,071)
    Balances as of June 30, 202458,519$— $1,699,127 $(735)$(1,207,253)$491,139 
    The accompanying notes are an integral part of these unaudited condensed consolidated financial statements.
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    Twist Bioscience Corporation
    Condensed Consolidated Statements of Cash Flows (unaudited)

    Nine Months Ended
    June 30,
    (in thousands) 20252024
    Operating activities
    Net loss$(50,532)$(174,071)
    Adjustments to reconcile net loss to net cash used in operating activities
    Depreciation and amortization expense18,84924,776
    Impairment of long-lived assets—44,930
    Stock-based compensation expense48,37638,578
    Gain on sale of business(48,847)—
    Other non-cash adjustments1,963462
    Changes in assets and liabilities:
    Accounts receivable, net [1]
    (15,172)11,398
    Inventories(1,861)3,578
    Prepaid expenses and other current assets [2]
    (1,822)(185)
    Other non-current assets(603)472
    Accounts payable
    9,616(6,927)
    Accrued expenses3,4344,132
    Accrued compensation(4,692)5,932
    Other liabilities[3]
    5,477(1,849)
    Net cash used in operating activities$(35,814)$(48,774)
    Investing activities
    Purchases of property and equipment$(15,577)$(3,074)
    Purchases of investments(28,257)(30,712)
    Proceeds from maturity of investments28,97532,000
    Proceeds from sale of business2,500—
    Net cash used in investing activities$(12,359)$(1,786)
    Financing activities
    Proceeds from exercise of stock options$5,551 $5,183 
    Proceeds from the issuance of liability related to sale of future revenue15,000—
    Proceeds from issuance of common stock under employee stock purchase plan2,4292,047
    Repurchases of common stock for income tax withholding(14)(3,967)
    Net cash provided by financing activities$22,966 $3,263 
    Effect of exchange rates on cash, cash equivalents and restricted cash$(20)$53 
    Net decrease in cash, cash equivalents, and restricted cash(25,227)(47,244)
    Cash, cash equivalents, and restricted cash at beginning of period229,132289,281
    Cash, cash equivalents, and restricted cash at end of period$203,905 $242,037 
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    Supplemental disclosure of cash flow information
    Income taxes paid, net of refunds$23 $363 
    Non-cash investing and financing activities
    Property and equipment additions included in accounts payable and accrued expenses$1,822 $226 
    Property and equipment capitalized acquired through tenant improvement allowance$— $2,719 
    Equity securities received as consideration for sale of business$53,890 $— 
    Promissory note receivable received as consideration for sale of business$1,696 $— 
    [1] Including changes in the related parties' balances of $(0.7) million and $0.9 million for the nine months ended June 30, 2025 and June 30, 2024, respectively.
    [2] Including changes in a related party balances of $(1.7) million and $— for the nine months ended June 30, 2025 and June 30, 2024, respectively.
    [3] Including changes in a related party' balances of $0.2 million and $— for the nine months ended June 30, 2025 and June 30, 2024, respectively.

    The accompanying notes are an integral part of these unaudited condensed consolidated financial statements.
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    Twist Bioscience Corporation
    Notes to Unaudited Condensed Consolidated Financial Statements
    1. Organization and Description of Business
    Twist Bioscience Corporation (the Company) was incorporated in the state of Delaware on February 4, 2013. The Company is a leading, rapidly growing synthetic biology company that has developed a disruptive DNA synthesis platform to industrialize the engineering of biology. The Company’s fiscal year ends on September 30.
    The core of the Company's platform is a proprietary technology that pioneers a new method of manufacturing synthetic DNA by “writing” DNA on a silicon chip. The Company has combined our silicon-based DNA writing technology with proprietary software, scalable commercial infrastructure and an e-commerce platform to create an integrated technology platform that enables us to achieve high levels of quality, precision, automation, and manufacturing throughput at a significantly lower cost than our competitors. The Company has applied its unique technology to manufacture a broad range of synthetic DNA-based products, including synthetic genes, tools for next generation sequencing, or NGS, sample preparation, and antibody libraries for drug discovery and development, all designed to enable its customers to conduct research more efficiently and effectively. The Company has leveraged the same technology to expand its footprint beyond DNA synthesis to manufacture synthetic RNA as well as antibody proteins to disrupt and innovate within larger market opportunities, in addition to discovery partnerships for biologic drugs.
    On May 2, 2025, the Company executed the Contribution Agreement (the "Contribution Agreement") pursuant to which the Company sold its DNA Data Storage business to Atlas Data Storage, Inc. ("Atlas"), a newly formed company, that will focus solely on DNA data storage technology and commercialization (see note 6).

    The Company has recognized annual losses from operations since inception and has an accumulated deficit of $1,292.4 million as of June 30, 2025. For the three months ended June 30, 2025, the Company reported net income of $20.4 million. The Company incurred net losses of $85.6 million for the same period in 2024, $50.5 million for the nine months ended June 30, 2025, and $174.1 million for the nine months ended June 30, 2024.
    As of June 30, 2025, the Company had cash and cash equivalents of $201.4 million and short-term investments of $49.4 million. The Company expects that its current cash, cash equivalents, and short-term investments will be sufficient to fund its operations for a period of at least one year from the date the condensed consolidated financial statements are issued.
    2. Summary of Significant Accounting Policies    
    Basis of Presentation
    The accompanying unaudited condensed consolidated financial statements were prepared in accordance with accounting principles generally accepted in the United States of America (GAAP) for interim financial information. Certain information and disclosures normally included in the consolidated financial statements prepared in accordance with GAAP have been condensed or omitted. Accordingly, these unaudited condensed consolidated financial statements should be read in conjunction with the audited consolidated financial statements and the related notes included in the Annual Report on Form 10-K for the fiscal year ended September 30, 2024 (the Annual Report on Form 10-K) filed with the Securities and Exchange Commission on November 18, 2024. The condensed consolidated financial statements are unaudited and have been prepared on a basis consistent with that used to prepare the audited annual consolidated financial statements and include, in the opinion of management, all adjustments, consisting of normal and recurring items, necessary for the fair statement of the condensed consolidated financial statements. The condensed consolidated balance sheet at September 30, 2024 is derived from audited consolidated financial statements but does not include all disclosures required by GAAP. The operating results for the three and nine months ended June 30, 2025 are not necessarily indicative of the results expected for the full year ending September 30, 2025 or any interim period. To conform with current period presentation, the Company reclassified $1.5 million of investment in equity securities included within other non-current assets to investment in equity securities in the condensed consolidated balance sheet as of September 30, 2024.
    Use of Estimates
    The presentation of unaudited condensed consolidated financial statements in conformity with GAAP requires management to make estimates and assumptions that affect the reported amounts of assets and liabilities and disclosure of contingent liabilities as of the date of the condensed consolidated financial statements and the
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    reported amounts of revenue and expenses during the reporting period. Actual results could differ from those estimates.
    Principles of Consolidation
    The Company’s unaudited condensed consolidated financial statements include its wholly owned subsidiaries. All intercompany balances and accounts are eliminated in consolidation.
    Cash and Cash Equivalents and Restricted Cash
    The following table provides a reconciliation of the Company’s cash and cash equivalents and non-current portion of restricted cash reported within the unaudited condensed consolidated balance sheets that sum to the total cash, cash equivalents and restricted cash shown in the Company’s condensed consolidated statements of cash flows:

    (in thousands)June 30, 2025September 30, 2024
    Cash and cash equivalents$201,373 $226,316 
    Restricted cash, non-current2,532 2,816 
    Total cash, cash equivalents and restricted cash$203,905 $229,132 
    Significant Accounting Policies
    There have been no material changes in the accounting policies from those disclosed in the audited consolidated financial statements and the related notes included in the Annual Report on Form 10-K other than disclosed below.
    Liability Related to the Sale of Future Revenue

    The Company accounts for the proceeds received from the monetization of future milestone and royalty payments from its contracts with certain customers as a debt instrument, which is amortized using the effective interest rate method over the estimated term of the arrangement. The Company recognizes interest expense thereon using the effective rate, which is based on its current estimates of future milestone and royalty payments under its customer contracts to be paid to the counterparty over the term of the arrangement. The Company periodically assesses these future estimated payments to impute interest on the carrying value of the liability. To the extent its estimates of future payments to the counterparty are greater or less than previous estimates or the estimated timing of such payments is materially different than previous estimates, the Company will account for any such changes by adjusting the effective interest rate on a prospective basis, with a corresponding impact to the liability. The assumptions used in determining the expected repayment term of the liability also requires that the Company makes estimates that could impact the classification of the liability, interest recorded on such liability, as well as the period over which such interest will be incurred. For further discussion, please see Note 16, Liability related to the sale of future revenue liability.

    Investment in equity securities

    The Company determines at the inception of each arrangement whether an investment or other interest is considered a variable interest entity (“VIE”). If the investment or other interest is determined to be a VIE, the Company evaluates whether it is considered the primary beneficiary. The primary beneficiary of a VIE is the party that meets both of the following criteria: (i) has the power to direct the activities that most significantly impact the VIE’s economic performance; and (ii) has the obligation to absorb losses or the right to receive benefits from the VIE. For investments in VIEs in which the Company is considered the primary beneficiary, the assets, liabilities and results of operations of the VIE are included in the Company’s consolidated financial statements.

    For investments in common stock or in-substance common stock where the Company has significant influence over the financial and operating policies of the investee are accounted for as equity-method investments.

    If the Company's equity investment do not meet the requirements of equity method of accounting, the Company accounts for the investment at fair value and has elected to account for its equity investments without a readily
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    determinable fair value using a measurement alternative. Equity investments without readily determinable fair values are recorded using the measurement alternative of cost less impairment, if any, adjusted for observable price changes in orderly transactions for identical or similar investments of the same issuer. Any impairments or adjustments are recorded in Other (income) expense, net on our consolidated statements of operations and comprehensive income (loss).

    Contingent Consideration

    The Company elected to account for the contingent consideration receivable under the Contribution Agreement with Atlas as a gain contingency in accordance with ASC 450, Contingencies (Subtopic 450-30). Under this approach, the Company recognizes the contingent consideration receivable in earnings after the contingency is resolved. Accordingly, to determine the initial gain on the sale of business, the Company did not include any amount related to the contingent consideration arrangement as part of the consideration received.

    Recent Accounting Pronouncements
    New Accounting Pronouncements Not Yet Adopted
    In December 2023, the FASB issued ASU No. 2023-09 "Income Taxes (Topic 740)". The amendments in this ASU require that public business entities on an annual basis (1) disclose specific categories in the rate reconciliation and (2) provide additional information for reconciling items that meet a quantitative threshold. The amendments in this update are effective for annual periods beginning after December 15, 2024. The standard is not expected to have a material impact to the Company's consolidated financial statements.
    In November 2023, the FASB issued ASU No. 2023-07 "Segment Reporting (Topic 280)". The amendments in this ASU improve reportable segment disclosure requirements, primarily through enhanced disclosures about significant segment expenses. The amendments in this update are effective for fiscal years beginning after December 15, 2023, and interim periods within fiscal years beginning after December 15, 2024, with early adoption permitted, and should be applied retrospectively. The Company will adopt ASU 2023-07 for the year ending September 30, 2025. The standard is not expected to have a material impact to the Company's consolidated financial statements.
    In November 2024, the FASB issued ASU 2024-03, Income Statement—Reporting Comprehensive Income—Expense Disaggregation Disclosures, which will require additional expense disclosures for all public entities. The amendments require that at each interim and annual reporting period, an entity will disclose certain disaggregated expenses included in each relevant expense caption, as well as the total amount of selling expenses and, in annual periods, an entity’s definition of selling expenses. ASU 2024-03 is effective for fiscal years beginning after December 15, 2026, and interim periods within fiscal years beginning after December 15, 2027, with early adoption permitted. The Company is currently evaluating the incremental disclosures that will be required in its financial statements.
    The Company has evaluated other recently issued accounting pronouncements and has concluded that the impact of recently issued standards that are not yet effective will not have a material impact on the Company’s financial position or results of operations upon adoption.
    3. Revenue
    The Company’s revenue is generated through the sale of synthetic biology tools, such as synthetic genes, oligo pools, NGS tools, DNA libraries and biopharma services for antibody discovery, optimization and development ("Biopharma").
    Contract Balances
    The following table summarizes our contract balances:
    (in thousands) June 30,
    2025
    September 30,
    2024
    Contract assets(1)
    $1,036 $2,031 
    Contract liabilities(2)
    6,234 2,131 
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    (1) Consists of unbilled amounts primarily related to Biopharma contracts which consists of research and development agreements with third parties.
    (2) Consists of receipt of advance payments before our performance obligations related to revenue contracts are met.
    For the three and nine months ended June 30, 2025, the Company recognized revenue of $1.7 million and $1.4 million, respectively, from the amount that was included in the contract liability balance at the beginning of each period. For the three and nine months ended June 30, 2024, the Company recognized revenue of $0.7 million and $2.9 million, respectively, from the amount that was included in the contract liability balance at the beginning of each period.
    In addition, for all periods presented, there was no revenue recognized in a reporting period from performance obligations satisfied in previous periods. The aggregate amount of the transaction price allocated to the performance obligations that are unsatisfied as of June 30, 2025 was $13.4 million. The Company expects to recognize revenue over the next twelve months relating to performance obligations unsatisfied as of June 30, 2025.
    Based on the nature of the Company’s contracts with customers which are recognized over a term of less than 12 months, the Company has elected to use the practical expedient whereby costs to obtain a contract are expensed as they are incurred. The Company states its revenues net of any taxes collected from customers that are required to be remitted to various government agencies. The amount of taxes collected from customers and payable to governmental entities is included on the balance sheet as part of “Accrued expenses and other current liabilities.”
    Disaggregation of Revenues

    The table below sets forth revenues by geographic region, based on ship-to destinations. Americas consists of the United States, Canada, Mexico and South America; EMEA consists of Europe, the Middle East, and Africa; and APAC consists of Japan, China, South Korea, India, Singapore, Malaysia and Australia.

    Three Months Ended
    June 30,
    Nine Months Ended
    June 30,
    (in thousands)2025202420252024
    Americas$59,387 $51,389 $168,285 $141,221 
    EMEA30,74223,58189,68867,064
    APAC5,9286,49419,59019,979
    Total$96,057 $81,464 $277,563 $228,264 

    The table below sets forth revenues by products.
    Three Months Ended
    June 30,
    Nine Months Ended
    June 30,
    (in thousands)2025202420252024
    Synthetic genes$27,680 $24,948 $82,387 $67,031 
    Oligo pools5,4234,16615,22812,295
    DNA libraries2,0563,8487,86710,318
    Antibody discovery5,6145,10217,00915,029
    NGS tools55,28443,400155,072123,591
    Total$96,057 $81,464 $277,563 $228,264 
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    The table below sets forth revenues by industry.

    Three Months Ended
    June 30,
    Nine Months Ended
    June 30,
    (in thousands)2025202420252024
    Industrial chemicals/materials$23,103 $23,188 $67,101 $59,730 
    Academic research15,93114,89250,00442,387
    Healthcare56,37542,823158,510124,637
    Food/agricultural6485611,9481,510
    Total$96,057 $81,464 $277,563 $228,264 

    4. Cash, Cash Equivalents and Investments
    The following table sets forth the cash and cash equivalents, and investments as of June 30, 2025:
    (in thousands)Amortized costGross unrealized gainsGross unrealized lossesFair value
    Cash$33,400 $— $— $33,400 
    Cash equivalents - money market funds167,973 — — 167,973 
    Total cash and cash equivalents$201,373 $— $— $201,373 
    Short-term investments:
    U.S. government treasury bills$49,427 $— $(2)$49,425 
    Total short-term investments$49,427 $— $(2)$49,425 
    Investment in equity securities of privately held companies$54,337 $— $— $54,337 
    Total cash, cash equivalents and investments$305,137 $— $(2)$305,135 

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    The following table sets forth the cash and cash equivalents, and investments as of September 30, 2024:
    (in thousands)Amortized costGross unrealized gainsGross unrealized lossesFair value
    Cash$26,458 $— $— $26,458 
    Cash equivalents - money market funds199,858 — — 199,858 
    Total cash and cash equivalents$226,316 $— $— $226,316 
    Short-term investments:
    U.S. government treasury bills$49,964 $119 $— $50,083 
    Total short-term investments$49,964 $119 $— $50,083 
    Investment in equity securities of privately held company$1,525 $— $— $1,525 
    Total cash, cash equivalents and investments$277,805 $119 $— $277,924 
    During the nine months ended June 30, 2025 and 2024, gross realized gains and losses related to our short-term investments were not material.

    During the nine months ended June 30, 2025 and 2024, the Company did not recognize any credit losses.

    5. Fair Value Measurement
    The Company assesses the fair value of financial instruments based on the provisions of ASC 820, Fair Value Measurements. ASC 820 defines fair value as the exchange price that would be received for an asset or paid to transfer a liability (an exit price) in the principal or most advantageous market for the asset or liability in an orderly transaction between market participants on the measurement date. ASC 820 also establishes a fair value hierarchy which requires an entity to maximize the use of observable inputs and minimize the use of unobservable inputs when measuring fair value. The standard describes three levels of inputs that may be used to measure fair value:
    Level 1—Quoted prices in active markets for identical assets or liabilities.
    Level 2—Observable inputs other than Level 1 prices such as quoted prices for similar assets or liabilities, quoted prices in markets that are not active, or other inputs that are observable or can be corroborated by observable market data for substantially the full term of the assets or liabilities.
    Level 3—Unobservable inputs that are supported by little or no market activity and that are significant to the fair value of the assets or liabilities.
    In determining fair value, the Company utilizes valuation techniques that maximize the use of observable inputs and minimize the use of unobservable inputs to the extent possible as well as considering counterparty credit risk in its assessment of fair value.
    Assets Measured at Fair Value on a Recurring Basis
    As of June 30, 2025, financial assets measured and recognized at fair value are as follows:

    (in thousands)Level 1Level 2Level 3Fair value
    Assets   
    Money market funds$167,973 $— $— $167,973 
    U.S. government treasury bills49,425 — — 49,425 
    Total financial assets$217,398 $— $— $217,398 

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    As of September 30, 2024, financial assets measured and recognized at fair value are as follows:

    (in thousands)Level 1Level 2Level 3Fair value
    Assets    
    Money market funds$199,858 $— $— $199,858 
    U.S. government treasury bills50,083— — 50,083
    Total financial assets$249,941 $— $— $249,941 

    Contractual maturities of short-term investments, as of June 30, 2025, were less than 12 months. The Company does not intend to sell the money market funds and short term investments and it is not more likely than not that the Company will be required to sell the investments before recovery of their amortized cost basis. Accrued interest receivable balances included in the prepaid expenses and other current assets within the condensed consolidated balance sheets were $1.2 million and $1.0 million as of June 30, 2025 and September 30, 2024, respectively.

    As of June 30, 2025 and September 30, 2024, there were no financial liabilities measured and recognized at fair value.
    There were no transfers between Level 1, Level 2 and Level 3 in the periods presented.

    6. Investment in Equity Securities
    On May 2, 2025, the Company executed the Contribution Agreement with Atlas for the sale and transfer of its DNA digital data storage assets, including the related intellectual property, equipment and contracts and the license of certain other intellectual property for a consideration of 73.0 million shares of Series Seed-1 Preferred Stock of Atlas, upfront cash consideration of $2.5 million, promissory notes of $2.0 million, contingent manufacturing and commercial milestone payments of up to $75.0 million, and royalty payments based on a percentage of Atlas sales of the DNA data storage products or services. As part of this transaction, certain employees of the Company were transitioned to Atlas.
    The Company concluded that its investment in shares of preferred stock of Atlas are not in substance common stock and accounted for the investment at fair value at its acquisition date of $53.9 million. The Company measured the fair value of our investment in Series Seed-1 Preferred Stock of Atlas using the backsolve method with consideration for a lack of marketability. The backsolve method was used to solve for the implied total equity value based on the recent Series Seed financing round by Atlas to third-party investors. Consideration was given to the rights and preferences of each of the classes of equity of Atlas and the expected time to a liquidity event. An option pricing allocation method, or OPM, was selected to allocate the total equity value. The following table lists the assumptions used to calculate the fair value of the investment:
    As of May 2,
    2025
    Discount for lack of marketability 10.0 %
    Risk-free interest rate3.8 %
    Time of exit3 years
    Volatility76.5 %
    Since Atlas is a private company, these securities do not have readily determinable fair value. Therefore, the Company elected to account for its investment in the preferred stock of Atlas using the measurement alternative method.
    The promissory notes are secured by the patents owned by Atlas and any income and royalties from such patents. The promissory note together with accrued interest at an interest rate of 4% per annum, is payable on the earliest of: (i) at Atlas' election, on or after December 31, 2028, (ii) the closing of the issuance and sale of shares of common stock in Atlas' first underwritten public offering, (iii) the 10 year anniversary from May 2, 2025, or (iv) Atlas’ insolvency, dissolution, or bankruptcy.
    The Company accounted for the promissory note receivable at its fair value of $1.7 million and recognized immaterial interest income on the promissory notes during the three and nine months ended June 30, 2025. Promissory note receivable is carried at amortized cost and reduced by a valuation allowance for estimated
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    credit losses, as necessary. The Company recognizes interest income on loans, including the amortization of discounts and premiums, using the effective interest method. As of June 30, 2025, the carrying value of the promissory note receivable is $1.7 million.
    The sale of DNA digital data storage assets to Atlas constitutes a disposition of a business. The Company concluded the disposition does not represent a strategic shift, and therefore, the Company has not accounted for the disposition as a discontinued operation. The Company recorded a $48.8 million gain on sale of business on the condensed consolidated statements of operations and comprehensive income (loss) for the three and nine months ended June 30, 2025. The Company allocated goodwill to the DNA Data Storage business disposed and the remaining business based on their relative fair values. The gain consists of the following assets transferred in accordance with the Contribution Agreement:

    (in thousands)As of May 2,
    2025
    Sale consideration:
    Fair value of Series Seed-1 Preferred Stock$53,890 
    Fair value of promissory note receivable1,696 
    Upfront cash consideration2,500 
    $58,086 
    Net assets sold:
    Property and equipment$(5,623)
    Goodwill(3,616)
    $(9,239)
    Gain on sale of business$48,847 
    The Company concluded that Atlas is a variable interest entity. While the Company holds one of the seven board of director seats in Atlas, the Company does not have the power, whether through contractual relationships or other factors, to direct the activities that most significantly impact the economic performance of Atlas. Therefore, the Company concluded it is not its primary beneficiary. The Company’s maximum exposure to loss from this VIE consist of investment in equity securities, promissory note receivable and other receivables for amounts billed under the transition services agreement and the sublease agreement aggregating to $57.4 million. There were no such impairments during the nine months ended June 30, 2025.
    The Company agreed to provide transitional support services to Atlas in the areas of accounting, payroll, and IT in return for payment on a time and material basis to Atlas in furtherance of its obligations under the Transition Services Agreement ("TSA") executed as part of the Contribution Agreement. The TSA also provides for continued access to relevant systems and other relevant premises on a transitional basis and reimbursement of third-party vendor costs for procurement of goods and services on behalf of Atlas. The Company's obligations under the TSA are expected to be completed within a year from the transaction date.
    Further, as part of the Contribution Agreement, the Company subleased specific office and lab space to Atlas in South San Francisco. The Company received a security deposit of $0.2 million for the sublease agreement. The Company recognized approximately $0.4 million of sublease income for the three and nine months ended June 30, 2025.
    The Company holds an equity investment in another privately held company which is accounted for as an equity security without a readily determinable fair value using a measurement alternative. The privately held company is a VIE, but the Company is not the primary beneficiary. The Company does not have the power to direct the activities that most significantly impact the economic performance of the investee. The Company’s maximum exposure to loss from this VIE consist of an equity investment of $0.4 million. The Company impaired $1.1 million during the nine months ended June 30, 2025. There was no such impairment during the nine months ended June 30, 2024.



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    7. Balance Sheet Components
    Allowance for Credit Losses

    Allowance for credit losses related to accounts receivable were $0.5 million and $0.7 million as of June 30, 2025 and September 30, 2024, respectively.

    Inventories

    Inventories consist of the following:
    (in thousands)June 30,
    2025
    September 30,
    2024
    Raw materials$17,497 $17,316 
    Work-in-process1,9602,146
    Finished goods6,4814,616
    $25,938 $24,078 
    There is no consigned inventory balance as of June 30, 2025 and September 30, 2024.
    Property and Equipment, net
    Property and equipment, net consists of the following:
    (in thousands) June 30,
    2025
    September 30,
    2024
    Laboratory equipment$96,443 $99,528 
    Furniture, fixtures and other equipment2,942 2,944 
    Vehicles211 211 
    Computer equipment3,160 3,249 
    Computer software10,270 10,095 
    Leasehold improvements58,273 57,448 
    Construction in progress13,334 4,688 
    $184,633 $178,163 
    Less: Accumulated depreciation and amortization(89,282)(75,643)
    $95,351 $102,520 

    Construction in progress mainly represents equipment costs, leasehold improvements costs and internal use software development costs. For the three and nine months ended June 30, 2025, the total depreciation and amortization expense was $5.8 million and $18.1 million, respectively. For the three and nine months ended June 30, 2024, the total depreciation and amortization expense was $7.0 million and $20.9 million, respectively. The Company disposed $5.6 million of property and equipment as part of the sale of DNA data storage business (see note 6).

    Other current liabilities
    Other current liabilities consist of the following:
    (in thousands)June 30,
    2025
    September 30,
    2024
    Income and other taxes payable$4,253$2,725
    Contract liabilities6,3242,131
    Other current liabilities500961
    $11,077 $5,817 
    8. Goodwill and Intangible Assets
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    The goodwill balance is presented below:
    (in thousands)June 30,
    2025
    September 30,
    2024
    Balance at beginning of period/year$85,811 $85,811 
    Sale of business (see note 6)(3,616)— 
    Balance at end of period/year $82,195 $85,811 
    The finite-lived intangible assets balances are presented below:

    June 30, 2025
    (in thousands, except for years)Weighted average
    Amortization period
    in years
    Gross
    carrying
    amount
    Accumulated
    amortization
    Net book
    value
    Developed Technology17$19,120 $(5,432)$13,688 
    Total finite-lived intangible assets$19,120 $(5,432)$13,688 

    September 30, 2024
    (in thousands, except for years)Weighted average
    Amortization period
    in years
    Gross
    carrying
    amount
    ImpairmentAccumulated
    amortization
    Net book
    value
    Developed Technology17$50,020 $(25,198)$(10,344)$14,478 
    Customer Relationships—15,210 (10,541)(4,669)— 
    Tradenames & Trademarks—900 (125)(775)— 
    Total finite-lived intangible assets$66,130 $(35,864)$(15,788)$14,478 

    Total amortization expense related to intangible assets was $0.3 million for the three months ended June 30, 2025 and $1.3 million for the three months ended June 30, 2024. Total amortization expense related to intangible assets was $0.8 million for the nine months ended June 30, 2025 and $3.9 million for the nine months ended June 30, 2024.
    9. Leases
    The Company leases certain of its facilities under non-cancellable operating leases expiring at various dates through 2044. The Company is also responsible for utilities, maintenance, insurance, and property taxes under these leases. The lease payments consist primarily of fixed rental payments for the right to use the underlying leased assets over the lease terms, as well as payments for common-area-maintenance and administrative services. The Company often receives customary incentives from its landlords, such as reimbursements for tenant improvements and rent abatement periods, which effectively reduce the total lease payments owed for these leases. Leases are classified as operating or financing at commencement. The Company does not have any material financing leases.
    Certain leases include options to renew or terminate at the Company’s discretion. The lease terms include periods covered by these options if it is reasonably certain the Company will renew or not terminate. The Company’s lease agreements do not contain any material residual value guarantees or restrictive covenants.
    As part of the sale of DNA data storage business, the Company subleased specific office and lab space to Atlas in South San Francisco (see note 6).
    Future minimum lease payments under all non-cancelable operating leases as of June 30, 2025 are as follows:

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    (in thousands)Operating
    leases
    Years ending September 30:
    Remainder of 2025
    $3,858 
    202613,963
    20278,450
    20288,510
    20296,609
    Thereafter81,405
    Total minimum lease payments$122,795 
    Less: imputed interest(44,645)
    Total operating lease liabilities$78,150 
    Less: current portion(15,501)
    Operating lease liabilities, net of current portion$62,649 

    The components of lease expense and supplemental information were as follows:
    Three Months Ended
    June 30,
    Nine Months Ended
    June 30,
    (in thousands except years and percentage)2025202420252024
    Operating lease costs$3,823 $3,800 $11,306 $11,800 
    Variable lease costs$2,012 $1,961 $5,820 $5,777 
    Sublease income $(417)$— $(417)$— 
    Weighted-average remaining lease term (in years) - operating leases15.215.2
    Weighted-average discount rate - operating leases6.5 %6.53 %
    Supplemental cash flow information related to leases are as follows:
    Nine Months Ended
    June 30,
    (in thousands)20252024
    Cash payments included in the measurement of operating lease liabilities
    $10,995 $11,100 
    10. Commitments and Contingencies
    Legal Proceedings
    The Company may be subject to litigation, claims and disputes in the ordinary course of business. Certain significant matters are described below. The Company recognizes accruals for matters to the extent that the Company concludes that a loss is both probable and reasonably estimable. If the Company determines that a material loss is reasonably possible and the loss or range of loss can be estimated, the Company discloses the possible loss.
    Securities Class Action
    On December 12, 2022, a putative securities class action lawsuit captioned Peters v. Twist Bioscience Corporation, et al., Case No. 22-cv-08168 (N.D. Cal.) (“Securities Class Action”) was filed in federal court in the Northern District of California (“Court”) against the Company, its Chief Executive Officer, and its Chief Financial Officer (the “Defendants”) alleging violations of federal securities laws. The Securities Class Action’s claims are based in large part on allegations made in a report issued on November 15, 2022 by Scorpion Capital (“Scorpion Report”) concerning, among other things, the Company’s DNA chip technology and accounting
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    practices. The initial complaint filed in the Securities Class Action alleges that various statements that the defendants made between December 13, 2019 and November 14, 2022 were materially false and misleading in light of the allegations in the Scorpion Report. The plaintiff who initiated the lawsuit sought to represent a class of shareholders who acquired shares of the Company’s common stock between December 13, 2019 and November 14, 2022 and sought damages as well as certain other costs. On July 28, 2023, the Court appointed a new plaintiff, not the original plaintiff who filed the case, as lead plaintiff in the case and appointed a new law firm as lead counsel. On October 11, 2023, the lead plaintiff filed an amended complaint. The amended complaint is purportedly brought on behalf of all persons other than the Defendants who acquired the Company’s securities between December 20, 2018 and November 15, 2022. The amended complaint alleges that certain statements regarding, among other things, the Company’s DNA products and accounting practices were false and misleading.
    This case remains in the preliminary stage. Given the inherent uncertainty of litigation and the legal standards that must be met, including class certification and success on the merits, the Company cannot express an opinion on the likelihood of an unfavorable outcome or on the amount or range of any potential loss. The Company and the other defendants intend to vigorously defend themselves against the claims asserted against them and filed a motion to dismiss the amended complaint on December 6, 2023. A hearing on the motion to dismiss was held on November 13, 2024 and the Company is now awaiting the judge's decision.

    Derivative Action

    On September 25, 2023, a shareholder derivative suit captioned Shumacher vs. Leproust et al., No. 1:23-cv-01048-UNA, was filed in the United States District Court for the District of Delaware against directors of the Company and an employee (the “Derivative Action”). The suit is based on substantially the same allegations in the Securities Class Action and seeks to recover, on behalf of the Company, damages to the Company arising from, among other things, the Securities Class Action. On November 13, 2023, the parties to the Derivative Action entered into a stipulation staying the Derivative Action pending resolution of the anticipated motion to dismiss the defendants have filed in the Securities Class Action.

    11. Related Party Transactions
    During the three and nine months ended June 30, 2025, the Company had revenues from related parties in the amount of $2.8 million and $9.6 million, respectively. During the three and nine months ended June 30, 2024, the Company had revenues from related parties in the amount of $4.1 million and $9.7 million, respectively.
    During the three and nine months ended June 30, 2025, there were no purchases of raw materials from related parties. During the three and nine months ended June 30, 2024, the Company purchased raw materials from related parties in the amount of $1.7 million and $4.5 million, respectively.
    As of June 30, 2025 and September 30, 2024 accounts receivable balances with related parties were $1.2 million and $0.5 million, respectively.
    As of June 30, 2025 and September 30, 2024, accounts payable balances with related parties were immaterial.
    Subsequent to the investment in Atlas, the Company concluded that Atlas is a related party as the Company has significant influence over Atlas but does not apply equity method of accounting because its investment is not in common stock or in-substance common stock of Atlas. The following are the transactions with Atlas:
    During the three and nine months ended June 30, 2025, the Company received sublease income accounted for as other income in the amount of $0.4 million and $0.4 million, respectively.

    As of June 30, 2025 other receivable balance related to the transition services agreement and sublease income (see note 6) included in prepaid and other current assets was $1.7 million.

    As of June 30, 2025, the carrying value of the promissory note receivable included in other non-current assets was $1.7 million. The Company recognized immaterial amount of interest income during the three and nine months ended June 30, 2025.

    As of June 30, 2025 sublease security deposit balance included in other non-current liabilities was $0.2 million.



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    12. Income Taxes
    In determining quarterly provisions for income taxes, the Company uses the annual estimated effective tax rate applied to the actual year-to-date profit or loss, adjusted for discrete items arising in that quarter. For the three and nine months ended June 30, 2025, the Company recorded provisions for income taxes of $0.2 million and $0.5 million, respectively. For the three and nine months ended June 30, 2024, the Company recorded provisions for income taxes of $0.2 million and $0.7 million, respectively.

    The sale of the Company's DNA digital data storage business is intended to comply with Section 351 of the Internal Revenue Code and the Company recognized taxable gain to the extent of the value of “boot” received. Because of carryover tax attributes (definite lived net operating losses) and valuation allowance against its US federal and states deferred tax assets, there was no impact on the Company’s provision for income taxes for the three and nine months ended June 30, 2025.

    On July 4, 2025, H.R.1, commonly referred to as the One Big Beautiful Bill Act, was enacted in the U.S., which includes a broad range of tax reform provisions, including extending and modifying certain key Tax Cuts and Jobs Act provisions (both domestic and international). The legislation has multiple effective dates, with certain provisions effective in 2025 and others to be implemented through 2027. While we are evaluating the full effects of the legislation we expect that the legislation will not have a material impact on our financial statements. As the legislation was signed into law after June 30, 2025, it had no impact on our operating results for the three and nine months ended June 30, 2025.

    13. Common Stock
    As of June 30, 2025, the Company had reserved sufficient shares of common stock, with a par value of $0.00001 per share, for issuance upon exercise of outstanding stock options. Each share of common stock is entitled to one vote. The holders of shares of common stock are also entitled to receive dividends whenever funds are legally available and when declared by the board of directors.

    In February 2025, the Company amended its Amended and Restated Certificate of Incorporation to provide for an increase in its authorized share capital. The authorized common stock increased to 200.0 million shares at a par value of $0.00001 per share.

    14. Stock-based Compensation
    In February 2025, as approved at the Annual Shareholders meeting, the Company amended its 2018 Equity Incentive Plan (the “Plan”), to increase the authorized shares issuable under the Plan by 3.7 million shares and eliminate the “evergreen” provision which provides for an automatic annual increase in the number of shares reserved for issuance of future awards under the Plan.
    Stock-based Compensation Expense
    The Company recorded stock-based compensation expense in the condensed consolidated statement of operations and comprehensive loss for the periods presented as follows (in thousands):

    Three Months Ended
    June 30,
    Nine Months Ended
    June 30,
    (in thousands)2025202420252024
    Cost of revenues$2,061 $1,033 $5,340 $3,006 
    Research and development9212,4007,1888,614
    Selling, general and administrative13,07510,30135,84826,958
    Total stock-based compensation expense$16,057 $13,734 $48,376 $38,578 

    An immaterial amount of stock-based compensation expense was capitalized to property and equipment related to capitalized software development costs for the three and nine months ended June 30, 2025 and 2024.

    Restricted Stock Units

    A summary of the Company’s restricted stock unit activity excluding the performance-based restricted stock units during the nine months ended June 30, 2025 was as follows:
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    (in thousands, except per share data)SharesWeighted average grant date fair value per share
    Nonvested shares at September 30, 20241,964$32.13 
    Granted1,18746.55
    Vested/Issued(725)39.20
    Forfeited(354)34.08
    Nonvested shares at June 30, 20252,072$37.58 

    As of June 30, 2025, there was $72.6 million of total unrecognized compensation cost related to these awards that is expected to be recognized over a weighted average period of 2.8 years. The total grant date fair value of the restricted stock units excluding performance-based restricted units awarded during the nine months ended June 30, 2025 was $55.2 million.
    Performance-based Restricted Stock Units
    During the nine months ended June 30, 2025, the Company granted additional performance-based restricted stock units to executives and employees that will vest upon achievement of multiple year revenue, gross margin, and profitability metrics as determined by the board of directors. Stock compensation expense for these awards is recorded over the vesting period based on the grant date fair value of the awards and probability of the achievement of specified performance targets. The grant date fair value is equal to the closing share price of the Company’s common stock on the date of grant. These awards will vest from a minimum of 10 months to a maximum of 34 months service period following the grant date, provided that the recipient is a Company employee at the time of vesting and the performance targets applicable to each award are achieved. The percentage of these awards that vest will depend on the achievement of specified performance targets at the end of the performance period and can range from 0% to 140% of the number of units granted.

    A summary of the Company’s performance-based restricted stock units activity during the nine months ended June 30, 2025 was as follows:

    (in thousands, except per share data)SharesWeighted average grant date fair value per share
    Nonvested shares at September 30, 20241,166$27.01 
    Granted1,40147.24
    Vested/Issued(311)44.90
    Forfeited(313)31.72
    Nonvested shares at June 30, 20251,943$37.97 

    As of June 30, 2025, the unrecognized compensation costs related to these awards was $45.2 million based on the maximum achievement of the performance targets. The Company expects to recognize those costs over a weighted average period of 1.7 years. The total grant date fair value of performance-based restricted stock units awarded during the nine months ended June 30, 2025 was $66.2 million.

    Stock Options
    A summary of the Company’s stock options including the performance-based stock options during the nine months ended June 30, 2025 was as follows:

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    (in thousands, except per share data)SharesWeighted average exercise price per shareWeighted average remaining contractual term (years)Aggregate intrinsic value
    Outstanding at September 30, 20241,892 $29.60 4.57$37,840 
    Forfeited(100)54.63— — 
    Exercised(329)16.85— 8,700 
    Outstanding at June 30, 20251,463 $30.77 3.83$17,537 
    Vested and exercisable at June 30, 20251,462 $30.73 3.83$17,537 
    As of June 30, 2025, the unrecognized compensation costs related to these awards was immaterial. The Company expects to recognize those costs over a weighted average period of 0.3 years.
    2018 Employee Stock Purchase Plan
    The number of shares of the Company's common stock reserved for issuance under the 2018 Employee Stock Purchase Plan ("ESPP") at June 30, 2025 was as follows:

    (In thousands)Shares
    available
    Outstanding at September 30, 2024
    611
    Additional shares authorized249
    Shares issued during the period(65)
    Outstanding at June 30, 2025
    795

    During the three and nine months ended June 30, 2025, ESPP expenses of $0.6 million and $1.7 million, respectively were recognized. During the three and nine months ended June 30, 2024, ESPP expenses of $0.5 million and $1.3 million, respectively were recognized.

    15. Net Income (Loss) Per Share
    The following table sets forth the computation of the Company’s basic and diluted net loss per share:

    Three Months Ended June 30,
    (in thousands, except per share data)20252024
    Numerator:
    Net income (loss)$20,390 $(85,571)
    Denominator:
    Weighted average shares used in computing net income (loss) per share, basic59,99558,145
    Effect of dilutive securities:
    Stock options483—
    Restricted stock units124—
    Performance-based restricted stock units548—
    Employee stock purchase plan 14—
    Weighted average shares used in computing net income (loss) per share, diluted61,16458,145
    Net income (loss) per share, basic$0.34 $(1.47)
    Net income (loss) per share, diluted$0.33 $(1.47)


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    Nine Months Ended
    June 30,
    (in thousands, except per share data)20252024
    Numerator:
    Net loss$(50,532)$(174,071)
    Denominator:
    Weighted average shares used in computing net income (loss) per share, basic and diluted 59,60057,806
    Net loss per share, basic and diluted $(0.85)$(3.01)

    The potentially dilutive common shares that were excluded from the calculation of diluted net loss per share because their effect would have been antidilutive for the periods presented are as follows:

    Three Months Ended
    June 30,
    Nine Months Ended
    June 30,
    (in thousands)2025202420252024
    Shares subject to options (including performance options) to purchase common stock2492,0201,4632,020
    Unvested restricted stock units and performance stock units 3,4703,5524,0153,552
    Shares subject to employee stock purchase plan—867586
    Total3,7195,6585,5535,658

    16. Liability Related to the Sales of Future Revenue
    On October 21, 2024, the Company executed the Royalty Purchase Agreement with XOMA (US) LLC ("XOMA Royalty"). Under the Royalty Purchase Agreement, XOMA Royalty provided Twist Bioscience an upfront payment of $15.0 million in cash in exchange for the right to receive half of the future potential milestone and royalty payments resulting from certain antibody discovery and biopharma services agreements between the Company and its customers ("underlying customer agreements"). The terms of the underlying customer agreements provide for milestone and royalty payments to the Company contingent upon the Company's customers achieving certain clinical, regulatory, development and commercial milestones. Under the terms of the underlying customer agreements, the Company has significant continuing involvement as the Company has ongoing obligations such as services to support the generation of these milestone and royalty payments. As such, the Company applied the guidance under ASC 470, Debt, and recorded the upfront payment of $15.0 million as a liability related to the sale of future revenue, which will be amortized using the effective interest method over the estimated term of the Royalty Purchase agreement.
    As milestone and royalty payments are remitted to XOMA Royalty, the balance of the liability related to the sale of future revenue will be effectively repaid over the term of the Royalty Purchase Agreement. In addition, in accordance with ASC 470, Debt, the Company will account for any milestone and royalty payments received in the future as revenue.
    The estimate of the effective interest rate contains significant assumptions regarding the timing and amount of expected milestone and royalty payments, which are considered Level 3 fair value inputs, and impact the interest expense that will be recognized over the term of the Royalty Purchase Agreement. As of June 30, 2025, the Company's estimate of aggregate future milestone and royalty payments expected to be paid to XOMA Royalty over the term of the Royalty Purchase Agreement did not exceed $15.0 million, therefore, no imputed interest expense has been recorded during the nine months ended June 30, 2025.



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    17. Impairment of long-lived assets
    On June 30, 2024, the Company identified an impairment indicator with respect to an asset group associated with our antibody discovery services product line (“Biopharma asset group”) due to lower than forecasted revenues. Therefore, the Company performed a recoverability test of long-lived assets by comparing the Biopharma asset group's net book value to its future undiscounted net cash flows. The Company concluded that the carrying value of the Biopharma asset group was not recoverable as it exceeded the future undiscounted cash flows the assets are expected to generate from their use and eventual disposition.
    To measure the impairment loss, the Company estimated the fair value of the Biopharma asset group by applying a discounted cash flow method. In applying the discounted cash flow method, the Company made certain estimates and assumptions including, among others, the level and timing of revenues, costs and expenses, working capital and discount rate. The Company used a discount rate of approximately 14%, which considers the inherent risks associated with the Biopharma asset group.

    The allocated impairment loss to any individual long-lived asset within the long-lived asset group cannot reduce the carrying amount of that long-lived asset below its fair value. Accordingly, the Company determined the fair value of the long-lived assets within the Biopharma asset group based on their highest and best use. These assets include intangible assets, property and equipment, and right-of use assets. The Company estimated the fair value of the developed technology intangible asset and the customer relationships intangible asset using an excess earnings model (income approach). The Company estimated the fair value of the trade name intangible asset using a relief from royalty approach. In applying these valuation methods to the intangible assets, the Company made certain estimates and assumptions including the level and timing of expected cash flows and discount rate, which is consistent with the 14% discount rate noted above. The Company determined the fair value of property and equipment based on a market approach. The Company determined the fair value of the lease right-of-use assets by applying a present value technique to the estimated market rate rental cash flows.

    Assumptions used to determine the fair values of certain lease right-of-use assets included estimated market rent and the discount rate.

    These fair value measurements were based on significant inputs not observable in the market and thus represent a Level 3 measurement. The Company believes the level and timing of expected future cash flows appropriately reflects market participant assumptions.

    As a result of allocating the impairment, the Company recorded the following impairment charges, which are included in “Impairment of long-lived assets” on our condensed consolidated statements of operations and comprehensive loss for the three and nine months ended June 30, 2024:
    Three and Nine Months Ended
    (in thousands)2024
    Property and equipment $9,066 
    Finite-lived intangible assets 35,864 
    Total$44,930 
    * * * * *

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    Item 2. Management’s Discussion and Analysis of Financial Condition and Results of Operations
    You should read the following discussion and analysis of our financial condition and results of operations together with the unaudited condensed consolidated financial statements and related notes that are included elsewhere in this Form 10-Q and our Annual Report on Form 10-K. This discussion contains forward-looking statements based upon current plans, expectations and beliefs that involve risks and uncertainties. Our actual results may differ materially from those anticipated in these forward-looking statements as a result of various factors, including, but not limited to, those discussed in the section entitled “Risk Factors” and elsewhere in this Form 10-Q. In preparing this MD&A, we presume that readers have access to and have read the MD&A in our Annual Report on Form 10-K, pursuant to Instruction 2 to paragraph (b) of Item 303 of Regulation S-K.
    Overview
    We are a leading, rapidly growing synthetic biology company that has developed a disruptive DNA synthesis platform to industrialize the engineering of biology. The core of our platform is a proprietary technology that pioneers a new method of manufacturing synthetic DNA by “writing” DNA on a silicon chip. We have combined our silicon-based DNA writing technology with proprietary software, scalable commercial infrastructure and an e-commerce platform to create an integrated technology platform that enables us to achieve high levels of quality, precision, automation, and manufacturing throughput at a significantly lower cost than our competitors. We have applied our unique technology to manufacture a broad range of synthetic DNA-based products, including synthetic genes, tools for next generation sequencing, or NGS, sample preparation, and antibody libraries for drug discovery and development, all designed to enable our customers to conduct research more efficiently and effectively. Leveraging our same technology, we have expanded our footprint beyond DNA synthesis to manufacture synthetic RNA as well as antibody proteins to disrupt and innovate within larger market opportunities, in addition to discovery partnerships for biologic drugs.
    We believe our products enable a broad range of applications that may ultimately improve health and the sustainability of the planet across multiple industries including healthcare, chemicals/materials, food/agriculture, and academic research. We sell our synthetic DNA and synthetic DNA-based products to a customer base of approximately 3,562 customers annually across a broad range of industries. In order to address this diverse customer base, we employ a multi-channel strategy comprised of a direct sales force targeting synthetic DNA customers, a direct sales force focusing on the NGS market and an e-commerce platform that serves both commercial channels. We employ business development and sales representatives for our biopharma solutions as well. Our easy-to-use e-commerce platform allows customers to design, validate and place on-demand orders of customized DNA online, and enables them to receive real-time customized quotes for their products and track their order status through the manufacturing and delivery process. This is a critical part of our strategy to address our large markets and diverse customer base, as well as drive commercial productivity, enhance the customer experience, and promote loyalty.
    We currently generate revenue through our synthetic biology and NGS tools product lines as well as biopharma services for antibody discovery, optimization and development.
    Since our inception, we have incurred net losses each year. Our net income for the three months ended June 30, 2025 was $20.4 million due to sale of DNA Data Storage business compared to net loss of $85.6 million, which included impairment of long-lived assets of $44.9 million for the three months ended June 30, 2024. As of June 30, 2025, we have accumulated net deficit of $1,292.4 million and cash, cash equivalents and short-term investments of $250.8 million. Our ability to generate product revenue sufficient to achieve profitability will depend heavily on the success of our existing products and the development and commercialization of additional products in the synthetic biology and biologic drug industries as well as leveraging our investment in our manufacturing facility in Wilsonville, Oregon.
    We sold our DNA Data Storage business to Atlas Data Storage, Inc. ("Atlas"), a newly formed company, that will focus solely on DNA data storage technology and commercialization, with $155.0 million in seed financing round from third-party investors. The purpose of the transaction is to unlock value by accelerating data storage technology development and allowing each company to focus strategically on its unique products, customers and investors. Under the terms of the contribution agreement executed with Atlas, we assigned and licensed our DNA data storage technology to Atlas in exchange of receiving a minority ownership interest upon close, an upfront cash payment and a secured promissory note. We retain an ownership stake in Atlas and may participate in the upside of DNA data storage through future technology and commercial milestone payments, and a revenue share through royalties on future sales of Atlas’ products and services.
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    Financial highlights from three months ended June 30, 2025, compared to the three months ended June 30, 2024, include the following:
    •Increase in revenues of 18% to $96.1 million from $81.5 million, primarily due to growth in NGS tools and synthetic genes revenues;
    •Gross margin increased to 53.4% from 43.3% mainly due to increase in revenues and holding manufacturing costs relatively flat;

    •Loss from operations decreased to $30.1 million from $88.9 million primarily due to impairment of long-lived assets recorded in the three months ended June 30, 2024 and increases in revenues and gross profit in the three months ended June 30, 2025;
    •Net cash used in operating activities decreased to $1.4 million from $6.4 million.
    •Gain on the sale of DNA digital data storage business of $48.8 million.
    Results of Operations
    Comparison of the Three Months Ended June 30, 2025 and 2024
    Revenues
    Three Months Ended
    June 30,
    (in thousands, except percentages) 20252024Change%
    Revenues$96,057 $81,464 $14,593 18 %
    Revenues by Geography
    We have one reportable segment from the manufacturing of synthetic DNA products. The following table shows our revenues by geography, based on our customers’ shipping addresses. Americas consists of United States, Canada, Mexico and South America; EMEA consists of Europe, Middle East and Africa; and APAC consists of Japan, China, South Korea, India, Singapore, Malaysia, Australia, New Zealand, Thailand and Taiwan.

    Three Months Ended June 30,
    (in thousands, except percentages)2025% 2024%
    Americas$59,387 62 %$51,389 61 %
    EMEA30,74232 %23,58130 %
    APAC5,9286 %6,4949 %
    Total revenues$96,057 100 %$81,464 100 %

    Revenues by Product
    The table below summarized revenues by product:

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    Three Months Ended June 30,
    (in thousands, except percentages)2025% 2024%
    Synthetic genes$27,680 29 %$24,948 29 %
    Oligo pools5,423 6 %4,1666 %
    DNA libraries2,056 2 %3,8485 %
    Antibody discovery5,614 6 %5,1026 %
    NGS tools55,284 57 %43,40054 %
    Total revenues$96,057 100 %$81,464 100 %

    Revenues by Industry
    The table below summarized revenues by industry:

    Three Months Ended June 30,
    (in thousands, except percentages)2025% 2024%
    Industrial chemicals/materials$23,103 24 %$23,188 27 %
    Academic research15,931 17 %14,89218 %
    Healthcare56,375 58 %42,82354 %
    Food/agriculture648 1 %5611 %
    Total revenues$96,057 100 %$81,464 100 %
    Product Shipments Including Synthetic Genes
    The table below summarized product shipments including synthetic genes:

    Three Months Ended June 30,
    June 30,March 31December 31,September 30,June 30,March 31,December 31,September 30,
    (in thousands)20252025202420242024202420232023
    Number of genes shipped237227205196212193171177

    The number of customers who purchased products from us were approximately 2,484 and 2,300 customers for the three months ended June 30, 2025 and 2024, respectively.
    Revenues increased 18% to $96.1 million for the three months ended June 30, 2025, as compared to $81.5 million for the three months ended June 30, 2024. The increase in revenues primarily reflects growth in NGS tools revenue of $11.9 million and growth in synthetic genes revenue of $2.7 million, which is primarily attributable to increase in revenues from our customers in the healthcare and academic research industries and an increase in the number of customers. The number of our genes shipped in the three months ended June 30, 2025 increased to approximately 237,000 genes compared to approximately 212,000 genes in the three months ended June 30, 2024, an increase of 12%.
    Cost of Revenues

    Three Months Ended
    June 30,
    (in thousands, except percentages)20252024Change%
    Cost of revenues$44,760 $46,193 $(1,433)(3)%
    Gross profit $51,297 $35,271 $16,026 45 %
    Gross margin53.4 %43.3 %10.1 %
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    Cost of revenues decreased 3% to $44.8 million for the three months ended June 30, 2025, as compared to $46.2 million for the three months ended June 30, 2024. The decrease is primarily attributable to a decrease in depreciation and amortization expense of $1.7 million due to impairment of long-lived assets in fiscal year 2024, a decrease in production overheads including facilities, IT services, and laboratory supplies of $0.5 million and a decrease in material costs of $0.3 million. This decrease was partially offset by an increase in stock-based compensation expense of $1.0 million. Gross margin increased 10.1% to 53.4% for the three months ended June 30, 2025, as compared to 43.3% for the three months ended June 30, 2024, mainly due to increase in revenues and holding manufacturing costs relatively flat and driving additional cost savings through continuous process improvement.

    Research and Development Expenses
    Three Months Ended
    June 30,
    (in thousands, except percentages)20252024Change%
    Research and development$18,047 $22,469 $(4,422)(20)%

    Research and development expenses decreased 20% to $18.0 million for the three months ended June 30, 2025, as compared to $22.5 million for the three months ended June 30, 2024. The decrease is primarily due to decreases in personnel costs of $2.4 million, stock-based compensation expenses of $1.5 million and depreciation expense of $0.6 million. The reduction in personnel expense and stock-based compensation expense is mainly due to the transition of the DNA digital data storage employees related to the sale of DNA digital data storage business during three months ended June 30, 2025.
    Selling, General and Administrative Expenses
    Three Months Ended
    June 30,
    (in thousands, except percentages)20252024Change%
    Selling, general and administrative$63,370 $56,794 $6,576 12 %

    Selling, general and administrative expenses increased 12% to $63.4 million for the three months ended June 30, 2025, as compared to $56.8 million for the three months ended June 30, 2024. The increase is primarily due to increases in stock-based compensation expense of $2.8 million, personnel costs of $1.9 million, marketing costs of $1.3 million, and IT services costs of $1.0 million. This increase was offset by decrease in other third party spending of $1.0 million.

    Impairment of long-lived assets
    Three Months Ended
    June 30,
    (in thousands, except percentages)20252024Change%
    Impairment of long-lived assets$— $44,930 $(44,930)(100)%

    We recognized impairment of intangible assets and property and equipment of $44.9 million related to the Biopharma asset group during the three months ended June 30, 2024.

    Gain on Sale of business
    Three Months Ended
    June 30,
    (in thousands, except percentages)20252024Change%
    Gain on sale of business$48,847 $— $48,847 N/A

    We recognized gain on sale of business of $48.8 million related to the sale of our DNA digital data storage business during three months ended June 30, 2025.

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    Interest and Other Income (Expense), net
    Three Months Ended
    June 30,
    (in thousands, except percentages)20252024Change%
    Interest income$2,690 $3,663 $(973)(27)%
    Other income (expense), net(836)(121)(715)591 %
    Total interest and other income (expense), net$1,854 $3,542 $(1,688)(48)%

    Interest income decreased 27% to $2.7 million for the three months ended June 30, 2025, as compared to $3.7 million for the three months ended June 30, 2024, due to lower cash equivalents and short-term investments balance.
    Income Tax Expense
    Three Months Ended
    June 30,
    (in thousands, except percentages)20252024Change%
    Income tax expense$(191)$(191)$— — %

    We recorded an income tax provision of $0.2 million and $0.2 million for the three months ended June 30, 2025 and June 30, 2024, respectively.


    Comparison of the Nine Months Ended June 30, 2025 and 2024
    Revenues
    Nine Months Ended
    June 30,
    (in thousands, except percentages) 20252024Change%
    Revenues$277,563 $228,264 $49,299 22 %
    Revenues by Geography
    We have one reportable segment from the manufacturing of synthetic DNA products. The following table shows our revenues by geography, based on our customers’ shipping addresses. Americas consists of United States, Canada, Mexico and South America; EMEA consists of Europe, Middle East and Africa; and APAC consists of Japan, China, South Korea, India, Singapore, Malaysia, Australia, New Zealand, Thailand and Taiwan.

    Nine Months Ended June 30,
    (in thousands, except percentages)2025%2024%
    Americas$168,285 61 %$141,221 62 %
    EMEA89,68832 %67,06429 %
    APAC19,5907 %19,9799 %
    Total revenues$277,563 100 %$228,264 100 %

    Revenues by Product
    The table below summarized revenues by product:

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    Nine Months Ended June 30,
    (in thousands, except percentages)2025%2024%
    Synthetic genes$82,387 30 %$67,031 28 %
    Oligo pools15,228 6 %12,295 6 %
    DNA libraries7,867 3 %10,318 4 %
    Antibody discovery17,009 6 %15,029 7 %
    NGS tools155,072 55 %123,591 55 %
    Total revenues$277,563 100 %$228,264 100 %

    Revenues by Industry
    The table below summarized revenues by industry:

    Nine Months Ended June 30,
    (in thousands, except percentages)2025%2024%
    Industrial chemicals/materials$67,101 24 %$59,730 25 %
    Academic research50,004 18 %42,38718 %
    Healthcare158,510 57 %124,63756 %
    Food/agriculture1,948 1 %1,5101 %
    Total revenues$277,563 100 %$228,264 100 %
    Revenues increased 22% to $277.6 million for the nine months ended June 30, 2025, as compared to $228.3 million for the nine months ended June 30, 2024. The increase in revenues primarily reflects growth in NGS tools revenue of $31.5 million and growth in synthetic genes revenue of $15.4 million, which is primarily attributable to an increase in revenues from our customers in the healthcare, industrial chemicals/materials and academic research industries and an increase in the number of customers. The number of our genes shipped in the nine months ended June 30, 2025 increased to approximately 669,000 genes compared to approximately 576,000 genes in the nine months ended June 30, 2024, an increase of 16%.
    Cost of Revenues

    Nine Months Ended
    June 30,
    (in thousands, except percentages)20252024Change%
    Cost of revenues$137,398 $133,148 $4,250 3 %
    Gross profit $140,165 $95,116 $45,049 47 %
    Gross margin50.5 %41.7 %8.8 %

    Cost of revenues increased 3% to $137.4 million for the nine months ended June 30, 2025, as compared to $133.1 million for the nine months ended June 30, 2024. The increase is primarily attributable to increases in material costs of $7.3 million due to higher sales volume, stock-based compensation expense of $2.3 million and production overheads costs including facilities, IT services, and laboratory supplies of $0.8 million. The increase was partially offset by decreases in depreciation and amortization expense of $4.4 million due to impairment of long-lived assets in fiscal year 2024 and outside services costs of $1.7 million. Gross margin increased 8.8% to 50.5% for the nine months ended June 30, 2025, as compared to 41.7% for the nine months ended June 30, 2024, mainly due to increase in revenues and holding manufacturing costs relatively flat and driving additional cost savings through continuous process improvement..

    Research and Development Expenses
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    Nine Months Ended
    June 30,
    (in thousands, except percentages)20252024Change%
    Research and development$63,271 $69,718 $(6,447)(9)%

    Research and development expenses decreased 9% to $63.3 million for the nine months ended June 30, 2025, as compared to $69.7 million for the nine months ended June 30, 2024. The decrease is primarily due to a decrease in personnel costs of $3.3 million, stock-based compensation expense of $1.4 million and depreciation expense of $1.5 million. The reduction in personnel expense and stock-based compensation expense is mainly due to the transition of the DNA digital data storage employees related to the sale of DNA digital data storage business during nine months ended June 30, 2025.
    Selling, General and Administrative Expenses
    Nine Months Ended
    June 30,
    (in thousands, except percentages)20252024Change%
    Selling, general and administrative$183,219 $165,256 $17,963 11 %

    Selling, general and administrative expenses increased 11% to $183.2 million for the nine months ended June 30, 2025, as compared to $165.3 million for the nine months ended June 30, 2024. The increase is primarily due to an increase in stock-based compensation expense of $8.9 million, personnel costs of $3.8 million, marketing costs of $1.2 million and other third party spending of $2.5 million.

    Impairment of long-lived assets
    Nine Months Ended
    June 30,
    (in thousands, except percentages)20252024Change%
    Impairment of long-lived assets$— $44,930 $(44,930)(100)%

    We recognized impairment of intangible assets and property and equipment of $44.9 million related to the Biopharma asset group during the nine months ended June 30, 2024.


    Gain on Sale of business
    Nine Months Ended
    June 30,
    (in thousands, except percentages)20252024Change%
    Gain on sale of business$48,847 $— $48,847 N/A

    We recognized gain on sale of business of $48.8 million related to sale of DNA digital data storage business during nine months ended June 30, 2025.

    Interest and Other Income (Expense), net
    Nine Months Ended
    June 30,
    (in thousands, except percentages)20252024Change%
    Interest income$8,731 $11,724 $(2,993)(26)%
    Other expense(1,323)(351)(972)277 %
    Total interest and other income (expense), net$7,408 $11,373 $(3,965)(35)%

    Interest income decreased 26% to $8.7 million for the nine months ended June 30, 2025, as compared to $11.7 million for the nine months ended June 30, 2024, due to lower cash equivalents and short-term investments balance.
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    Income Tax Expense
    Nine Months Ended
    June 30,
    (in thousands, except percentages)20252024Change%
    Income tax expense$(462)$(656)$194 (30)%

    We recorded an income tax provision of $0.5 million and $0.7 million for the nine months ended June 30, 2025 and June 30, 2024, respectively.


    Liquidity and Capital Resources
    Sources of Liquidity
    To date, we have financed our operations principally through public equity raises, private placements of our convertible preferred stock, borrowings from credit facilities and revenue from our commercial operations. At June 30, 2025, we had a balance of $201.4 million of cash and cash equivalents and $49.4 million in short-term investments.
    During the nine months ended June 30, 2025, we received an upfront payment of $15.0 million from XOMA (US) LLC ("XOMA") in cash in exchange for XOMA's right to receive half of the future potential milestone and royalty payments resulting from existing antibody discovery and biopharma services collaborations with the Company’s partners. We accounted for the transaction as liability for the sale of future revenue under the accounting guidance for sale of future revenues (see note 15 of the condensed consolidated financial statements included elsewhere in this Form 10-Q).
    On May 2, 2025, the Company executed the Contribution Agreement with Atlas for the sale and transfer of its DNA digital data storage assets including the related intellectual property, equipment and contracts and the license of certain other intellectual property and the license of certain other intellectual property for a consideration of 73.0 million shares of Series Seed-1 Preferred Shares of Atlas, upfront cash consideration of $2.5 million, promissory notes of $2.0 million issued by Atlas, contingent manufacturing and commercial milestone payments of up to $75.0 million, and royalty payments based on a percentage of Atlas sales of the DNA data storage products (see note 15 of the condensed consolidated financial statements included elsewhere in this Form 10-Q).
    Operating Capital Requirements
    Our primary uses of capital are, and we expect will continue to be for the near future, compensation and related expenses, manufacturing costs, laboratory and related supplies, legal and other regulatory expenses and general overhead costs and capital expenditures. We had $10.3 million in commitments for capital expenditures as of June 30, 2025.

    Cash Flows
    The following table summarizes our sources and uses of cash and cash equivalents:

    Nine Months Ended
    June 30,
    (in thousands)20252024
    Net cash used in operating activities$(35,814)$(48,774)
    Net cash provided by (used in) investing activities(12,359)(1,786)
    Net cash provided by financing activities22,966 3,263 

    Operating Activities
    Net cash used in operating activities was $35.8 million during the nine months ended June 30, 2025 primarily due to a net loss of $50.5 million adjusted for non-cash items including depreciation and amortization expense
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    of $18.8 million, stock-based compensation expense of $48.4 million, gain on sale of business of $48.8 million and a net cash outflow from operating assets and liabilities of $5.6 million. The net cash outflow from operating assets and liabilities was mainly due to an increase in accounts receivable of $15.2 million due to the increase in revenues and the timing of collections, increases in inventories of $1.9 million and prepaid and other current assets of $1.8 million, a decrease in accrued compensation of $4.7 million offset by increases in accounts payable of $9.6 million, other liabilities of $5.5 million and accrued expenses of $3.4 million. The changes in accrued compensation, accrued expenses, and accounts payable are due to timing of payments to vendors and employees.

    Net cash used in operating activities was $48.8 million during the nine months ended June 30, 2024 and consisted primarily of a net loss of $174.1 million adjusted for non-cash items including depreciation and amortization expense of $24.8 million, stock-based compensation expense of $38.6 million, impairment of long-lived assets of $44.9 million and a net change in operating assets and liabilities of $16.6 million. The change in operating assets and liabilities was mainly due to decreases in accounts receivable of $11.4 million due to the timing of collections, inventories of $3.6 million, other non-current assets of $0.5 million, accounts payable of $6.9 million and other liabilities of $1.8 million offset by increases in prepaid expenses of $0.2 million, accrued compensation of $5.9 million and accrued expenses of $4.1 million.

    Investing Activities
    Net cash used in investing activities was $12.4 million during the nine months ended June 30, 2025, which consisted of the purchases of property and equipment of $15.6 million offset by the proceeds from the sale of our data storage business of $2.5 million and the net impact of purchases and maturity of investments of $0.7 million.
    Net cash used in investing activities was $1.8 million during the nine months ended June 30, 2024, which consisted of the purchases of laboratory property, equipment and computers of $3.1 million offset by the proceeds from the net impact of purchases and maturity of investments of $1.3 million.
    Financing Activities
    Net cash provided by financing activities was $23.0 million in the nine months ended June 30, 2025, which consisted of $15.0 million from XOMA for the sale of future revenue, $5.6 million from the exercise of stock options and $2.4 million proceeds from issuance of shares under the ESPP.
    Net cash provided by financing activities was $3.3 million in the nine months ended June 30, 2024, which consisted of $5.2 million from the exercise of stock options and $2.0 million proceeds from issuance of shares under the 2018 ESPP offset by $4.0 million in repurchases of common stock for income tax withholdings.
    Off-Balance Sheet Arrangements
    We do not have any off-balance sheet arrangements.
    Contractual Obligations and Other Commitments
    Our contractual obligations have not materially changed from those reported in our Annual Report on Form 10-K.
    Critical Accounting Policies and Significant Management Estimates
    The preparation of our Condensed Consolidated Financial Statements in accordance with generally accepted accounting principles in the United States of America requires management to make estimates and judgments that affect the reported amounts in the financial statements and related disclosures. On an ongoing basis, we evaluate our significant accounting policies and estimates. We base our estimates on historical experience and on various market-specific and other relevant assumptions that we believe to be reasonable under the circumstances, the results of which form the basis for making judgments about the carrying values of assets and liabilities that are not readily apparent from other sources. Estimates are assessed each period and updated to reflect current information. Actual results may differ significantly from these estimates. A summary of our critical accounting policies and estimates is presented in Part II, Item 7 of our Annual Report on Form 10-K. There were no changes to our critical accounting policies and estimates during the nine months ended June 30, 2025 other than the updates to the critical accounting policies and estimates set forth below.

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    Calculation of Gain on Sale of business
    On May 2, 2025, we measured the fair value of our investment in Series Seed-1 Preferred Stock of Atlas using the backsolve method with consideration for a lack of marketability. The backsolve method was used to solve for the implied total equity value based on the recent Series Seed financing round by Atlas to third-party investors. Consideration was given to the rights and preferences of each of the classes of equity of Atlas and the expected time to a liquidity event. An option pricing allocation method, or OPM, was selected to allocate the total equity value. The OPM treats ordinary shares and preferred shares as call options on the total equity value of a company, with exercise prices based on the value thresholds at which the allocation among the various holders of a company’s securities changes. Under this method, the Series Seed-1 Preferred Stock held by the Company will have value only if the funds available for distribution to shareholders exceeded the value of the Preferred Shares’ liquidation preference at the time of the liquidity event, such as a strategic sale or a merger.

    Equity investments held by the Company lack readily determinable fair values and therefore the securities are measured at cost minus impairment, if any, plus or minus changes resulting from observable price changes in orderly transactions for the identical or similar equity securities of the same issuer. The Company reviews the carrying value of its equity investments for impairment whenever events or changes in business circumstances indicate the carrying amount of such asset may not be fully recoverable. Impairments, if any, are based on the excess of the carrying amount over the recoverable amount of the asset.

    Recent Accounting Pronouncements
    For a description of accounting changes and recent accounting pronouncements, including the expected dates of adoption and estimated effects, if any, on our condensed consolidated financial statements, see Note 2, “Summary of significant accounting policies” in Item 1 of Part I of this Form 10-Q.

    Item 3. Quantitative and Qualitative Disclosures About Market Risk
    Information about our market risk is presented in Part II, Item 7A “Quantitative and Qualitative Disclosures about Market Risk” of our Annual Report on Form 10-K. Our exposure to market risk has not changed materially since September 30, 2024.
    Item 4. Controls and Procedures
    Evaluation of Disclosure Controls and Procedures
    Under the supervision and with the participation of our management, including our Chief Executive Officer and Chief Financial Officer, we evaluated the effectiveness of our disclosure controls and procedures (as that term is defined in Rules 13a-15(e) and 15d-15(e) under the Exchange Act) as of June 30, 2025, which is the end of the period covered by this Form 10-Q. Based on this evaluation, the Chief Executive Officer and Chief Financial Officer concluded that the Company’s disclosure controls and procedures to ensure that information required to be disclosed by the Company in reports we file or submit under the Exchange Act is (i) recorded, processed, summarized, evaluated and reported, as applicable, within the time periods specified in the SEC’s rules and forms and (ii) accumulated and communicated to the Company’s management, including the Company’s Chief Executive Officer and Chief Financial Officer, as appropriate to allow timely decisions regarding required disclosures were effective as of June 30, 2025.

    Changes in Internal Control over Financial Reporting
    There were no changes in our internal control over financial reporting identified in connection with the evaluation required by Rule 13a-15(d) and 15d-15(d) of the Exchange Act that occurred during the quarter ended June 30, 2025, that have materially affected, or are reasonably likely to materially affect, our internal control over financial reporting.

    Limitations on Effectiveness of Controls and Procedures

    Our disclosure controls and procedures are designed to provide reasonable assurance of achieving their objectives, as specified above. Our management recognizes that any control system, no matter how well designed and operated, is based upon certain judgments and assumptions and cannot provide absolute assurance that its objectives will be met.


    36

    Table of Contents
    PART II. OTHER INFORMATION

    Item 1. Legal Proceedings
    For a description of material pending legal proceedings, see Note 6 “Commitments and Contingencies - Legal Matters” of the Notes to Condensed Consolidated Financial Statements included in Part I, Item 1 of this Form 10-Q. In addition, we are subject to various legal proceedings and claims arising in the ordinary course of business. Although occasional adverse decisions or settlements may occur, management believes that the final disposition of such matters will not have a material adverse effect on our business, financial position, results of operations or cash flows.

    Item 1A. Risk Factors
    In addition to the other information set forth in this Quarterly Report on Form 10-Q, you should carefully consider the risk factors and other cautionary statements described under the heading “Item 1A. Risk Factors” included in our Annual Report on Form 10-K filed with the SEC on November 18, 2024, as updated and supplemented in Part II, “Item 1A. Risk Factors” in our Quarterly Report on Form 10-Q filed with the SEC on May 5, 2025, which could materially affect our business, financial condition or future results. Additional risks and uncertainties not currently known to us or that we currently deem to be immaterial also may materially and adversely affect our business, financial condition or future results. There have been no material changes in our risk factors from those described in our Annual Report on Form 10-K. We may disclose changes to risk factors or additional risk factors from time to time in our future filings with the SEC.

    Item 2. Unregistered Sales of Equity Securities and Use of Proceeds
    Sales of Unregistered Securities
    None.

    Item 3. Defaults Upon Senior Securities
    None.

    Item 4. Mine Safety Disclosures
    Not applicable.

    Item 5. Other Information
    Rule 10b5-1 Trading Plans
    On May 7, 2025, Dennis Cho, the Company's Chief Legal Officer and Corporate Secretary, adopted a trading plan intended to satisfy the affirmative defense conditions of Rule 10b5-1(c) under the Exchange Act (a “10b5-1 Plan”). Mr. Cho’s 10b5-1 Plan provides for the potential sale of up to 31,945 shares of the Company’s common stock and will expire on the earlier of May 15, 2026 or the date when all shares under Mr. Cho’s 10b5-1 Plan are sold.
    On May 8, 2025, Adam Laponis, the Company's Chief Financial Officer, adopted a 10b5-1 Plan. Mr. Laponis’ 10b5-1 Plan provides for the potential sale of up to 24,000 shares of the Company’s common stock and will expire on the earlier of August 20, 2026 or the date when all shares under Mr. Laponis’ 10b5-1 Plan are sold.
    On May 13, 2025, Patrick Finn, the Company's President & Chief Operating Officer, adopted a 10b5-1 Plan. Mr. Finn’s 10b5-1 Plan provides for the potential sale of up to 97,496 shares of the Company’s common stock and will expire on the earlier of May 13, 2026 or the date when all shares under Mr. Finn’s 10b5-1 Plan are sold.
    On May 27, 2025, Emily M. Leproust, the Company's Chief Executive Officer, adopted a 10b5-1 Plan. Dr. Leproust’s 10b5-1 Plan provides for the potential sale of up to 458,276 shares of the Company’s common stock
    37

    Table of Contents
    and will expire on the earlier of September 16, 2026 or the date when all shares under Dr. Leproust’s 10b5-1 Plan are sold.


    38

    Table of Contents
    Item 6. Exhibits

    Exhibit
    Number
    Description Filed / Furnished /
    Incorporated from
    Form
    Incorporated by Reference from Exhibit NumberDate Filed
    10.1*
    Contribution Agreement by and between Twist Bioscience Corporation and Altas Data Storage, Inc., dated May 2, 2025.
    Filed herewith
    10.2*
    License Agreement by and between Twist Bioscience Corporation and Atlas Data Storage, Inc., dated May 2, 2025.
    Filed herewith
    10.3*
    MES Software License Agreement by and between Twist Bioscience Corporation and Atlas Data Storage, Inc., dated May 2, 2025
    Filed herewith
    31.1
    Certification pursuant to Section 302 of the Sarbanes-Oxley Act of 2002, Rule 13(a)-14(a)/15d-14(a), by Chief Executive Officer.
    Filed herewith
    31.2
    Certification pursuant to Section 302 of the Sarbanes-Oxley Act of 2002, Rule 13(a)-14(a)/15d-14(a), by Chief Financial Officer.
    Filed herewith
    32.1†
    Certification pursuant to 18 U.S.C. Section 1350, as adopted pursuant to Section 906 of the Sarbanes-Oxley Act of 2002, by Chief Executive Officer.
    Furnished herewith
    32.2†
    Certification pursuant to 18 U.S.C. Section 1350, as adopted pursuant to Section 906 of the Sarbanes-Oxley Act of 2002, by Chief Financial Officer.
    Furnished herewith
    101
    The following materials from Twist Bioscience Corporation’s Quarterly Report on Form 10-Q for the quarter ended June 30, 2025, formatted in iXBRL (inline eXtensible Business Reporting Language): (i) the Condensed Consolidated Balance Sheets, (ii) the Condensed Consolidated Statements of Operations and Comprehensive Loss, (iii) the Condensed Consolidated Balance Sheets, (iv) the Condensed Consolidated Statements of Stockholders’ Equity, (v) the Condensed Consolidated Statements of Cash Flows, and (vi) Notes to Unaudited Condensed Consolidated Financial Statements, tagged as blocks of text.
    Filed herewith
    104
    The cover page from the Company’s Quarterly Report on Form 10-Q for the quarter ended June 30, 2025, formatted in Inline XBRL (included in Exhibit 101).
    Filed herewith
    39

    Table of Contents
    *Certain information in this exhibit and exhibits or schedules thereto has been omitted pursuant to Regulation S-K Item 601(a)(5) and/or Item 601(b)(10)(iv) because it is both (i) not material and (ii) the type that Twist Bioscience Corporation customarily and actually treats as private and confidential. An unredacted copy of the exhibit or schedule will be furnished on a supplemental basis to the SEC upon request; provided that Twist Bioscience Corporation may request confidential treatment for any exhibit or schedule so furnished.
    †The certifications attached as Exhibits 32.1 and 32.2 that accompany this Quarterly Report on Form 10-Q are not deemed filed with the SEC and are not to be incorporated by reference into any filing of Twist Bioscience Corporation under the Securities Act of 1933, as amended, or the Securities Exchange Act of 1934, as amended, whether made before or after the date of this Quarterly Report on Form 10-Q, regardless of any general incorporation language contained in any filing.

    40

    Table of Contents
    SIGNATURES
    Pursuant to the requirements of the Securities Exchange Act of 1934, the registrant has duly caused this report to be signed on its behalf by the undersigned thereunto duly authorized.

    August 4, 2025Twist Bioscience Corporation
    By:/s/ Adam Laponis
    Adam Laponis
    Chief Financial Officer
    (Authorized officer)


    41
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